WebJun 13, 2024 · Camptothecin is an anticancer molecule which acts by targeting the human deoxyribonucleic acid topoisomerase I (Topo-I). It inhibits the enzyme by blocking the re-joining step of the cleavage reaction of Topo-I, which causes accumulation of a covalent reaction intermediate. WebDNA single- strand breaks were detected using alkaline sucrose gradient centrifugation when HL-60 cells were incubated with 10 μMCPT or 10 μMSN-38 for 30 min. These …
Apoptosis and Its Modulation in Human Promyelocytic HL …
WebJun 1, 1992 · Camptothecin or teniposide induced apoptosis selectively in S phase of HL-60 cells; apoptotic cells from camptothecin-treated cultures, however, despite the marked nucleolysis, still expressed p120. WebFeb 1, 2008 · So, we conclude that rhPLD2 alone cannot induce apoptosis in HL-60 cells, but it can potentiate the apoptosis of HL-60 cells induced by camptothecin. Similarly, we show that both rhPLD2 and standard PLD were able to enhance camptothecin-induced apoptosis of HL-60 cells. Previousarticlein issue Nextarticlein issue Keywords rhPLD2 … ootp perfect team 22 forum
DNA Damage and Cell Killing by Camptothecin and Its …
WebAug 26, 2002 · Camptothecin (CAM; Sigma, St. Louis, MO) initiates apoptosis in the human promyelocytic leukemia cell line, HL-60, via its binding to topoisomerase I–DNA … WebJun 1, 2000 · Camptothecin (50 mM), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I, was used as a positive control (King et al., 2000). ... Internalization of Bacillus intermedius... WebCamptothecin [47, 48]. binds non-covalently with topoisomerase-I and suppresses their catalytic activity, thus show- Vinblastine and its derivatives have been used ing anti-cancer properties. ... (HL-60, MCF-7) with IC50 value Due to the large diversity of endophytic fungi, (5.8, 20.15 μM) [92]. Incarxanthone B, a novel various novel cytotoxic ... ootp perfect points sale