Peasy t1
WebMar 15, 2024 · Purified PCR products were cloned and constructed pEASY-T1-CGMMV, pEASY-T1- A. citrulli, and pEASY-T1- EF-1α (540-bp) plasmid by using a pEASY-T1 Simple Cloning Kit (TransGen Biotech, Beijing, China), respectively. WebMar 30, 2024 · Escherichia colicloning vector pEASY-T1, expression vector pEASY-E2, competent cells Trans1-T1 and Transetta (DE3) were purchased from TransGen Biotech (Beijing, China). The whole gene of the mature form of ANGPTL8/betatrophin was synthesized by Inovogen Tech. Co. (Beijing, China) into pUCE plasmids.
Peasy t1
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WebpEASY® -Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa. • 5 minutes fast ligation of Pfu-amplified PCR products. WebApr 24, 2015 · The reference plasmid pEASY-T1-STY2879 was constructed as follows: the full length STY2879 gene, which was from the S. Typhi strain CT18, was amplified by a set of primers, STY2879-F: 5’-3’ ttacaacttaaagagtttcgc and STY2879-R: 5’-3’ atgacaatagaagttcaaagg, and the PCR products (774 bp) were ligated into the pEASY-T1 …
WebPeasy T1 Simple Cloning Vector System, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebJan 31, 2014 · The EcoR I/Not I digested fragments of pEASY-T1-ADH1B were ligated with pPICZB, resulting in pPICZB-ADH1B. The recombined plasmid was transfected by electroporation of Pichia pastoris. Enzyme purification and assays. Cells were harvested by centrifugation and washed with 50 mM Tris–HCl, pH8.0 (buffer A). The cell paste was …
WebDec 6, 2024 · The tmexCD1.2-toprJ1 and tmexCD2.2-toprJ2 gene clusters were amplified by PCR and ligated to the pEasy-T1 vector (Transgen, Beijing, China) using Gibson cloning (Gibson Assembly Cloning Kit, NEB). The primers used for subcloning are listed in Table S1 (available as Supplementary data at JAC Online). WebpEASY® -T1 Simple Cloning Kit Catalog Number：CT111-01 Price：$97.24 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now …
WebpEASY ® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. ... •Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70°C for six months; others at -20 ...
WebAug 1, 2024 · A Positive positive reference plasmid for Brucella spp. detection (pEASY-T1- omp2a) was constructed by our lab. TwistAmp exo and TwistAmp basic Kits were provided by TwistDx (Cambridge, UK). DNase/RNase-Free distilled, deionized water (DDH2O) was provided by Tiangen Biochemical Co., Ltd. brown lug boots womenWebpEASY®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.•5 … every mother counts controversyWebpEASY-T3 (linearized) TA cloning vector that allows the cloned PCR product to be excised with EcoRI or NotI. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets No matches brown luggage leather satchel purseWebApr 12, 2024 · The first PCR product and the intermediate vector pEASY-T1-intron were digested by Sac I and Spe I, and then ligated after gel purification. The recombinant plasmid was transformed into DH5α, verified by double digestion with Hin d III and Eco R V, and then confirmed by sequencing. brown luke monicaWebpeasy 系列克隆载体; 相关产品; 克隆感受态细胞; 点突变; 核酸纯化 . 基因组dna纯化; 质粒dna纯化; dna产物纯化; rna纯化; 相关产品; 二代测序 . dna建库; rna建库; 相关产品; mngs; … brown l shaped sectional sofaWebpEASY® -T1 Cloning Kit Catalog Number：CT101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add to … brown low waisted pantsWebpEASY -Blunt Cloning Kit 使用前请务必仔细阅读说明书目录号：CB101 保存:Trans1-T1 Phage Resistant Chemically Competent Cell-70℃保存六个月，其它-20℃保存六个月。 *片段为胶回收产物，反应时间取最大值。 5、最佳反应温度：25℃ *若片段是高GC结构，可以37℃反应。 （推荐用PCR 仪控温） ® 试剂盒组成 pEASY- Blunt Cloning Vector (10 … brown l shape sofa bed