WebDownload scientific diagram Immature or mature DCs were pulsed with OVA/BSA coated beads for 5min, washed and separated into two samples. One sample was chased for … WebAnchana Rathinasamy. Hi Taghreed, if you do not know how efficient your Tumor cells are in the processing of ova and still unclear of how much time is required for pulsing, try one or 2 experiments by pulsing the Tumor cells with DQ-ovalbumin (BODIPY FL conjugated, invitrogen) and in parallel pulse DC with the same.
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WebWhen electroporated DCs were pulsed with OVA for additional times, both MHC class I- and II-restricted presentation of OVA were increased compared with each single procedure, including electroporation or simple pulse. Immunization with DCs loaded with OVA by electroporation induced higher cytotoxicity of splenocytes to E.G7 cells, a clone of ... WebMar 1, 1998 · It is shown that a single immunization with DC pulsed with OVA peptide was highly effective in eliciting a protective immune response against a challenge with tumor cells expressing the OVA gene, and that in vivo priming of CTL and induction of antitumor immunity by bone marrow-generated DC also require the presentation of MHC class II ... re-download ffxiv
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WebHalf of the splenocytes were pulsed with SIINFEKL (ovalbumin, OVA 257–264) (10 µg/mL) (AS-60 193-1, AnaSpec) for 2 hour at 37°C, 5% CO 2 and remaining cells were left unloaded. Cells loaded with SIINFEKL were labeled with 5 µM CTV and unloaded cells were labeled with 500 nM CTV. WebWe first fixed the OVA concentration at 100 μg/mL and examined the impact of adding various concentrations of DOTAP-CLs . When BMDCs were pulsed with 100 μg/mL of OVA in the presence of 20 μg/mL of DOTAP-CLs, we observed significantly enhanced cross-priming and expansion of OT-I CD8 + T-cells (P<0.05, Figure 3A and C). WebAug 6, 2014 · One, 3, and, 5 days after the tumor challenge, mice were injected intravenously with PBS, iDC, DCs-OVA (DCs pulsed with OVA 257–264 ), or Rv0652-DCs-OVA (Rv0652 … redownload final draft